© 2014 by John Wiley & Sons, Inc. Genome sequences and RNA-seq data provide unparalleled views into the genetic basis that underlies plant phenotypes. This chapter outlines a paradigm shift in plant biology originating from the relative ease of generating sequences, and points to tools that have enabled global transcript analyses (RNA-seq), replacing arduous microarray hybridization experiments and laborious quantitative RT-PCR platform designs. Soon, it can be envisioned that one may replace individual transcript assays by measuring how the entire transcriptome responds globally to a manipulation or mutation. The chapter advances a position that the knowledge of genome content and structure, and conditional transcript expression characteristics can explain a plant's growth and adaptation to the environment. This point is highlighted by comparing the genome and transcriptome of the abiotic stress-sensitive Arabidopsis thaliana with its close relatives, Thellungiella parvula and Thellungiella salsuginea.
Publication Source (Journal or Book title)
Plant Abiotic Stress, Second Edition
Oh, D., Dassanayake, M., Hong, H., George, S., Paeng, S., Kropornika, A., Bressan, R., Lee, S., Yun, D., & Bohnert, H. (2014). Genomics of plant abiotic stress tolerance. Plant Abiotic Stress, Second Edition, 231-255. https://doi.org/10.1002/9781118764374.ch9