Genomics of plant abiotic stress tolerance

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© 2014 by John Wiley & Sons, Inc. Genome sequences and RNA-seq data provide unparalleled views into the genetic basis that underlies plant phenotypes. This chapter outlines a paradigm shift in plant biology originating from the relative ease of generating sequences, and points to tools that have enabled global transcript analyses (RNA-seq), replacing arduous microarray hybridization experiments and laborious quantitative RT-PCR platform designs. Soon, it can be envisioned that one may replace individual transcript assays by measuring how the entire transcriptome responds globally to a manipulation or mutation. The chapter advances a position that the knowledge of genome content and structure, and conditional transcript expression characteristics can explain a plant's growth and adaptation to the environment. This point is highlighted by comparing the genome and transcriptome of the abiotic stress-sensitive Arabidopsis thaliana with its close relatives, Thellungiella parvula and Thellungiella salsuginea.

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Plant Abiotic Stress, Second Edition

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