Purification, crystallization and preliminary X-ray diffraction studies of retinal dehydrogenase type II
One enzyme which catalyzes the last step of the formation of the hormone retinoic acid from vitamin A (retinol) is retinal dehydrogenase type II (RalDH2). RalDH2, expressed in the Escherichia coil BL21(DE3) strain, was purified and crystallized using ammonium sulfate as a precipitant. These crystals belong to the space group P212121 (a = 108, b = 150, c = 168 Å, α = β = γ = 90°).
Publication Source (Journal or Book title)
Acta Crystallographica Section D: Biological Crystallography
Lamb, A., Wang, X., Napoli, J., & Newcomer, M. (1998). Purification, crystallization and preliminary X-ray diffraction studies of retinal dehydrogenase type II. Acta Crystallographica Section D: Biological Crystallography, 54 (4), 639-642. https://doi.org/10.1107/S0907444997014121