A helical lid converts a sulfotransferase to a dehydratase
We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.
Publication Source (Journal or Book title)
Nature Structural Biology
Pakhomova, S., Kobayashi, M., Buck, J., & Newcomer, M. (2001). A helical lid converts a sulfotransferase to a dehydratase. Nature Structural Biology, 8 (5), 447-451. https://doi.org/10.1038/87617