Title

A helical lid converts a sulfotransferase to a dehydratase

Document Type

Article

Publication Date

5-9-2001

Abstract

We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.

Publication Source (Journal or Book title)

Nature Structural Biology

First Page

447

Last Page

451

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