The structure of human 5-lipoxygenase
The synthesis of both proinflammatory leukotrienes and anti-inflammatory lipoxins requires the enzyme 5-lipoxygenase (5-LOX). 5-LOX activity is short-lived, apparently in part because of an intrinsic instability of the enzyme. We identified a 5-LOX-specific destabilizing sequence that is involved in orienting the carboxyl terminus, which binds the catalytic iron. Here, we report the crystal structure at 2.4 angstrom resolution of human 5-LOX stabilized by replacement of this sequence.
Publication Source (Journal or Book title)
Gilbert, N., Bartlett, S., Waight, M., Neau, D., Boeglin, W., Brash, A., & Newcomer, M. (2011). The structure of human 5-lipoxygenase. Science, 331 (6014), 217-219. https://doi.org/10.1126/science.1197203