© 2018 Elsevier Inc. Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4°C for 3 months with virtually no loss of activity. In addition, an engineered “pseudo wild-type” enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes.
Publication Source (Journal or Book title)
Methods in Enzymology
Gilbert, N., Neau, D., & Newcomer, M. (2018). Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla. Methods in Enzymology, 605, 33-49. https://doi.org/10.1016/bs.mie.2018.02.010