Title

Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla

Document Type

Article

Publication Date

1-1-2018

Abstract

© 2018 Elsevier Inc. Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4°C for 3 months with virtually no loss of activity. In addition, an engineered “pseudo wild-type” enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes.

Publication Source (Journal or Book title)

Methods in Enzymology

First Page

33

Last Page

49

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