Title

Universal primers for the amplification of chloroplast microsatellites in grasses (Poaceae)

Document Type

Article

Publication Date

6-1-2004

Abstract

Attempts to design truly universal primers to amplify chloroplast microsatellites have met with limited success due to nonconservation of repeat loci across widely divergent taxa. We have used the complete chloroplast genome sequences of rice, maize and wheat to design five pairs of primers that amplify homologous mononucleotide repeats across the Poaceae (grasses). Sequencing confirmed conservation of repeat motifs across subfamilies and a preliminary study in Anthoxanthum odoratum revealed polymorphism at two loci with a haplotype diversity value of 0.495. These primers provide a valuable tool to study cytoplasmic diversity in this extensively studied and economically important range of taxa.

Publication Source (Journal or Book title)

Molecular Ecology Notes

First Page

262

Last Page

264

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