Quantitative PCR for DNA identification based on genome-specific interspersed repetitive elements
We have designed and evaluated a series of class-specific (Aves), order-specific (Rodentia), and species-specific (equine, canine, feline, rat, hamster, guinea pig, and rabbit) polymerase chain reaction (PCR)-based assays for the identification and quantitation of DNA using amplification of genome-specific short and long interspersed elements. Using SYBR Green-based detection, the minimum effective quantitation levels of the assays ranged from 0.1 ng to 0.1 pg of starting DNA template. Background cross-amplification with DNA templates derived from sixteen other species was negligible prior to 30 cycles of PCR. The species-specificity of the PCR amplicons was further demonstrated by the ability of the assays to accurately detect known quantities of species-specific DNA from mixed (complex) sources. The 10 assays reported here will help facilitate the sensitive detection and quantitation of common domestic animal and bird species DNA from complex biomaterials. © 2003 Elsevier Inc. All rights reserved.
Publication Source (Journal or Book title)
Walker, J., Hughes, D., Hedges, D., Anders, B., Laborde, M., Shewale, J., Sinha, S., & Batzer, M. (2004). Quantitative PCR for DNA identification based on genome-specific interspersed repetitive elements. Genomics, 83 (3), 518-527. https://doi.org/10.1016/j.ygeno.2003.09.003