Steryl Glucosides in Phaseolus aureus. use of Gas-Liquid Chromatography and Mass Spectrometry for Structural Identification
Chloroform-methanol extracts of a membrane-containing particulate enzyme fraction from Phaseolus aureus were concentrated to a small volume and subjected to thin layer chromatography in three solvent systems. An α-naph-thol-H2SO4-staming band was purified and analyzed by gas-liquid chromatography and by combined gas-liquid chroma-tography-mass spectrometry. Two major components were identified as stigmasteryl and β-sitosteryl glucosides by mass spectra of the intact molecules as trimethylsilyl derivatives. © 1971, American Chemical Society. All rights reserved.
Publication Source (Journal or Book title)
Laine, R., & Elbein, A. (1971). Steryl Glucosides in Phaseolus aureus. use of Gas-Liquid Chromatography and Mass Spectrometry for Structural Identification. Biochemistry, 10 (13), 2547-2553. https://doi.org/10.1021/bi00789a020