Steryl Glucosides in Phaseolus aureus. use of Gas-Liquid Chromatography and Mass Spectrometry for Structural Identification
Abstract
Chloroform-methanol extracts of a membrane-containing particulate enzyme fraction from Phaseolus aureus were concentrated to a small volume and subjected to thin layer chromatography in three solvent systems. An α-naph-thol-H2SO4-staming band was purified and analyzed by gas-liquid chromatography and by combined gas-liquid chroma-tography-mass spectrometry. Two major components were identified as stigmasteryl and β-sitosteryl glucosides by mass spectra of the intact molecules as trimethylsilyl derivatives. © 1971, American Chemical Society. All rights reserved.
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