Cell-free biosynthesis of erythroglycan in a microsomal fraction from K-562 cells
Particulate membrane preparations from K-562 [human CML (chronic-myelogenous-leukaemia)-derived] cells catalyse the transfer of [3H]galactose from UDP-[3H]galactose and [3H]N-acetylglucosamine from UDP-[3H]N-acetylglucosamine into an endogenous product that on digestion with Pronase yields long-chain glycopeptides (mol. wt. 7000-10000) called 'erythroglycan'. Incorporation of either labelled sugar increased up to 60 min of incubation time. The labelled erythroglycan was isolated by chromatography on Sephadex G-50 and characterized by digestion with endo-β-galactosidase from Escherichia freundii, followed by analysis on Bio-Gel P-2 and paper chromatography. This digestion gave the following four products: (1) a disaccharide with the sequence βGlcNAc-βGal; (2) a trisaccharide with the sequence βGal-βGlcNAc-βGal; (3) a larger oligosaccharide containing galactose and N-acetylglucosamine; and (4) a putative protein-linkage region.
Publication Source (Journal or Book title)
Russin, T., Laine, R., & Turco, S. (1981). Cell-free biosynthesis of erythroglycan in a microsomal fraction from K-562 cells. Biochemical Journal, 197 (2), 327-332. https://doi.org/10.1042/bj1970327