Title

Carbohydrate Structures of Three Novel Phosphoinositol-Containing Sphingolipids from the Yeast Histoplasma capsulatum

Document Type

Article

Publication Date

1-1-1984

Abstract

From the yeast phase of the human pathogen Histoplasma capsulatum, three novel glycolipids were isolated, shown to react with sera from histoplasmosis patients, and partially characterized: compound V, ceramide-P-inositol-[mannose2]; compound VI, ceramide-P-inositol-[mannose2, galactose]; compound Vlll, an isomer of compound VI [Barr, K., & Lester, R. L. (1984) Biochemistry (preceding paper in this issue)]. Ammonolysis of these lipids has yielded all the carbohydrate (oligosaccharides V, VI, and VIII) as novel, intact oligosaccharides suitable for characterization. Anomeric configurations were determined by specific glycosidase digestion and by the stability of peracetylated saccharides to Cr03 oxidation. Linkages were established by methylation analysis. These experiments yielded the following structural assignments: manp(a1→3)manp(a1→2 or 6)myoinositol oligosaccharide V manp(a1→3) manp(a1→ 2 or 6)myoinositol galf(a1→6) oligosaccharide VI manp(a1→3)manp(a1→ 2 or 6)myoinositol galp(β1→4) oligosaccharide VIII The occurrence of galactofuranose is novel for glycosphingolipids, and it is noteworthy that compound VI is immuno-reactive. © 1984, American Chemical Society. All rights reserved.

Publication Source (Journal or Book title)

Biochemistry

First Page

5589

Last Page

5596

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