Liquid chromatography-electrospray ionization-mass spectrometric quantitation of juvenile hormone III in whole body extracts of the Formosan subterranean termite
Juvenile hormone (JH) III is responsible for control of a variety of insect physiological and developmental states, including caste differentiation of the Formosan subterranean termite (Coptotermes formosanus Shiraki). We report here a simplified, efficient sample preparation and an optimized LC-ESI-MS method for quantifying JH III in whole body extracts. Sample preparation comprises hexane extraction (from termite whole bodies) and C18 cartridge purification. Previous LC-ESI-MS protocols exhibited the following two problems: (1) ion fragmentation differed when comparing spectra from insect samples and authentic JH III and (2) a JH III monitoring ion was not resolved from other unknown compounds in whole body samples from termites. To overcome these problems, we used a pentafluorophenyl LC column and water/acetonitrile containing ammonium acetate as solvent. In a mass chromatogram (m/z 235) of termite samples, a peak was detected at the retention time of authentic JH III, and MS2 of this peak confirmed that the ion is a fragment of JH III, [M-CH3OH+H]+, being the base peak in both termites and authentic JH III samples. The protocol enables quantification of JH III in a single termite with signal/noise >10:1 and the limit of quantification is 21 pg. © 2009 Elsevier B.V. All rights reserved.
Publication Source (Journal or Book title)
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Miyazaki, M., Mao, L., Henderson, G., & Laine, R. (2009). Liquid chromatography-electrospray ionization-mass spectrometric quantitation of juvenile hormone III in whole body extracts of the Formosan subterranean termite. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877 (27), 3175-3180. https://doi.org/10.1016/j.jchromb.2009.08.008