An enzymatic synthesis of specifically radiolabelled derivatives of the common osmolyte, glycine betaine
A simple, rapid and efficient method has been developed for the synthesis of radiolabelled glycine betaine. Relatively inexpensive 14C- or 3H-labelled choline obtained from commercial sources is oxidized effectively using commercially available choline oxidase. Recoveries of >80% with >95% radiochemical purity are obtained using ion exchange chromatography. The addition of [14C-methyl]glycine betaine to samples from the water column of a hypersaline lake illustrate but one application for studies of methylated amine metabolism. © 1987.
Publication Source (Journal or Book title)
Journal of Experimental Marine Biology and Ecology
King, G. (1987). An enzymatic synthesis of specifically radiolabelled derivatives of the common osmolyte, glycine betaine. Journal of Experimental Marine Biology and Ecology, 107 (2), 145-154. https://doi.org/10.1016/0022-0981(87)90192-4