Ablation of Ezh2 in neural crest cells leads to aberrant enteric nervous system development in mice
© 2018 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In the current study, we examined the role of Ezh2 as an epigenetic modifier for the enteric neural crest cell development through H3K27me3. Ezh2 conditional null mice were viable up to birth, but died within the first hour of life. In addition to craniofacial defects, Ezh2 conditional null mice displayed reduced number of ganglion cells in the enteric nervous system. RT-PCR and ChIP assays indicated aberrant up-regulation of Zic1, Pax3, and Sox10 and loss of H3K27me3 marks in the promoter regions of these genes in the myenteric plexus. Overall, these results suggest that Ezh2 is an important epigenetic modifier for the enteric neural crest cell development through repression of Zic1, Pax3, and Sox10.
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Kim, H., Langohr, I., Faisal, M., McNulty, M., Thorn, C., & Kim, J. (2018). Ablation of Ezh2 in neural crest cells leads to aberrant enteric nervous system development in mice. PLoS ONE, 13 (8) https://doi.org/10.1371/journal.pone.0203391