Targeted gene replacement by homologous recombination in Drosophila stimulates production of second-site mutations
Gene replacement by homologous recombination is a powerful technique for generating mutations in Drosophila. While using this technique for the BEAF gene, we encountered non-targeted lethal mutations on the target chromosome that complicated the analysis of the BEAF mutations until they were discovered and removed by meiotic recombination. Subsequent experiments indicated that the gene-targeting method leads to a modest but significant three-fold increase in the rate of production of non-targeted lethal mutations. It is important to be aware of this phenomenon when using this method. © 2010 Landes Bioscience.
Publication Source (Journal or Book title)
Roy, S., & Hart, C. (2010). Targeted gene replacement by homologous recombination in Drosophila stimulates production of second-site mutations. Fly, 4 (1), 12-17. https://doi.org/10.4161/fly.4.1.10995