Title

Targeted gene replacement by homologous recombination in Drosophila stimulates production of second-site mutations

Document Type

Article

Publication Date

1-1-2010

Abstract

Gene replacement by homologous recombination is a powerful technique for generating mutations in Drosophila. While using this technique for the BEAF gene, we encountered non-targeted lethal mutations on the target chromosome that complicated the analysis of the BEAF mutations until they were discovered and removed by meiotic recombination. Subsequent experiments indicated that the gene-targeting method leads to a modest but significant three-fold increase in the rate of production of non-targeted lethal mutations. It is important to be aware of this phenomenon when using this method. © 2010 Landes Bioscience.

Publication Source (Journal or Book title)

Fly

First Page

12

Last Page

17

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