Cloning and functional characterization of LCR-F1: A bZIP transcription factor that activates erythroid-specific, human globin gene expression
DNase I hypersensitive site 2 (HS 2) of the human β-globin Locus Control Region (LCR) directs high level expression of the β-globin gene located 50 kilobases downstream. Experiments in cultured cells and in transgenic mice demonstrate that duplicated AP1-like sites in HS 2 are required for this powerful enhancer activity. A cDNA clone encoding a basic, leucine-zipper protein that binds to these sites was isolated and designated Locus Control Region-Factor 1 (LCR-F1). This protein is a member of a new family of regulatory factors that contain a 63 amino acid 'CNC domain' overlapping the basic region. This domain is approximately 70% identical in the Drosophila Cap N Collar (CNC) protein, NF-E2 and LCR-F1. LCR-F1 transactivates an HS 2/λ-globin reporter gene over 170-fold in transient transfection experiments specifically in erythroid cells. These results suggest that LCR-F1 may be a critical factor involved in LCR-mediated, human globin gene expression. © 1994 Oxford University Press.
Publication Source (Journal or Book title)
Nucleic Acids Research
Caterina, J., Donze, D., Sun, C., Ciavatta, D., & Townes, T. (1994). Cloning and functional characterization of LCR-F1: A bZIP transcription factor that activates erythroid-specific, human globin gene expression. Nucleic Acids Research, 22 (12), 2383-2391. https://doi.org/10.1093/nar/22.12.2383