Title

Density gradient enrichment of Escherichia coli conditional msbA mutants

Document Type

Article

Publication Date

12-1-2007

Abstract

Insight into the mechanism of lipid transport to the outer membrane of gram-negative bacteria has been hampered by the lack of an effective genetic screen for defective mutants. This work demonstrates an enrichment of conditional mutants defective in lipopolysaccharide export by Ludox density gradient centrifugation and selection for detergent resistance. New temperature-sensitive mutants with lipid export defects were isolated with single missense mutations in msbA. The results demonstrate the power of this approach for the study of lipid export in Escherichia coli. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Publication Source (Journal or Book title)

Applied and Environmental Microbiology

First Page

7992

Last Page

7996

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