Title

Stimulation of Escherichia coli DNA damage inducible DNA helicase DinG by the single-stranded DNA binding protein SSB

Document Type

Article

Publication Date

11-2-2012

Abstract

Escherichia coli DNA damage inducible protein DinG is a superfamily II DNA helicase and is closely related to human DNA helicase XPD. Here, we report that E. coli single-stranded DNA binding protein (SSB) is able to form a stable protein complex with DinG and to stimulate the DinG DNA helicase activity. An SSB mutant that retains the single-stranded DNA binding activity but fails to form a protein complex with DinG becomes a potent inhibitor for the DinG DNA helicase, suggesting that E. coli wild-type SSB stimulates the DinG DNA helicase via specific protein-protein interaction. Structured summary of protein interactions: SSB and SSB bind by molecular sieving (View interaction) DinG and SSB bind by molecular sieving (View interaction) DinG and SSB bind by cosedimentation in solution (View interaction) © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Publication Source (Journal or Book title)

FEBS Letters

First Page

3825

Last Page

3830

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