Title

Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging

Document Type

Article

Publication Date

1-1-2021

Abstract

We utilized collagen specific second harmonic generation (SHG) signatures coupled with correlative immunofluorescence imaging techniques to characterize collagen structural isoforms (type I and type III) in a murine model of myocardial infarction (MI). Tissue samples were imaged over a four week period using SHG, transmitted light microscopy and immunofluorescence imaging using fluorescently-labeled collagen antibodies. The post-mortem cardiac tissue imaging using SHG demonstrated a progressive increase in collagen deposition in the left ventricle (LV) post-MI. We were able to monitor structural morphology and LV remodeling parameters in terms of extent of LV dilation, stiffness and fiber dimensions in the infarcted myocardium.

Publication Source (Journal or Book title)

Biomedical optics express

First Page

604

Last Page

618

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