Acute osmotic tolerance of cultured cells of the oyster pathogen Perkinsus marinus (Apicomplexa : Perkinsida)
Cultured Perkinsus marinus cells were exposed for 24 hr to salinities of 0, 3, 6, 9, 12 and 22 ppt at temperatures of 1, 5, 10, 15 and 28°C in artificial seawater (ASW) and to the same salinities at 28°C in ASW with the osmotic concentration adjusted with sucrose to the equivalent of 22 ppt. At 28°C mortality increased as salinity decreased below 22 ppt. Mortality was greater than 99% at 0 ppt and greater than 90% at 3 ppt. Mortality was 70% at 6 ppt, 43% at 9 ppt and 20% at 12 ppt. Mortality was low (<5%) and equal to that at 22 ppt in all treatments where osmotic concentration was maintained with sucrose. Mortality occurred rapidly, within 5 min of exposure to experimental conditions. In the region where mortality was most sensitive to salinity changes (6-12 ppt), lower temperature caused an increase in mortality, but the temperature effect was significant only at 9 ppt. © 1994.
Publication Source (Journal or Book title)
Comparative Biochemistry and Physiology -- Part A: Physiology
Burreson, E., Ragone Calvo, L., La Peyre, J., Counts, F., & Paynter, K. (1994). Acute osmotic tolerance of cultured cells of the oyster pathogen Perkinsus marinus (Apicomplexa : Perkinsida). Comparative Biochemistry and Physiology -- Part A: Physiology, 109 (3), 575-582. https://doi.org/10.1016/0300-9629(94)90196-1