Title

Optimal culture conditions for the propagation of the oyster pathogen perkinsus marinus (apicomplexa) in protein deficient medium

Document Type

Article

Publication Date

1-1-1996

Abstract

The protozoan, Perkinsus marinus, acclimated and proliferated in the culture medium JL-ODRP-1 without bovine serum albumin. The principal culture conditions for the optima propagation of P. marinus in this protein deficient culture medium, were determined. The greatest growth rates of the parasite occurred at 28 °C, 661 mOsm/kg and pH 7.5. Decreasing seeding densities from 16 × 105 to 1 × 105 also increased growth rate. After several passages in the absence of 5 % CO2 tension, the growth rote of P. marinus was similar to its original value in the presence of 5 % CO2 tension. This protein deficient culture medium is ideally suited to study parasite-derived proteins since P. marinus extracellular proteins in culture supernatants were produced and were easily detected by gel electrophoresis and silver staining.

Publication Source (Journal or Book title)

Parasite

First Page

147

Last Page

153

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