Truncation of the carboxy-terminal 28 amino acids of glycoprotein B specified by herpes simplex virus type 1 mutant amb1511-7 causes extensive cell fusion
Three amber mutations were introduced proximal to the syn3 locus of the herpes simplex virus type 1 glycoprotein B (gB) gene specifying gB derivatives lacking the carboxy-terminal 28, 49, or 64 amino acids. A complementation system that utilized gBs expressed in COS cells to complement gB-null virus KΔT was established. The 49- or 64-amino-acid-truncated gBs failed to complement gB-null virus KΔT, while the 28-amino-acid-truncated gB complemented KΔT efficiently. Mutant herpes simplex virus type 1 KOS (amb1511-7) specifying the 28-amino-acid-truncated gB fused Vero cells extensively.
Publication Source (Journal or Book title)
Journal of Virology
Baghian, A., Huang, L., Newman, S., Jayachandra, S., & Kousoulas, K. (1993). Truncation of the carboxy-terminal 28 amino acids of glycoprotein B specified by herpes simplex virus type 1 mutant amb1511-7 causes extensive cell fusion. Journal of Virology, 67 (4), 2396-2401. https://doi.org/10.1128/jvi.67.4.2396-2401.1993