Document Type
Article
Publication Date
12-17-2001
Abstract
To facilitate detection of glycoprotein K (gK) specified by herpes simplex virus, a 12-amino-acid epitope tag was inserted within gK domain III. Recombinant virus gKprotC-DIII, expressing the tagged gK, was isolated. This virus formed wild-type plaques and replicated as efficiently as the wild-type KOS virus in Vero cells. Anti-protein C MAb detected high-mannose and Golgi complex-dependent glycosylated gK within cells as well as on purified virions. The gK-null virus ΔgK (gK-/-) entered Vero cells substantially more slowly than the wild-type KOS (gK+/+), while ΔgK virus grown in complementing VK302 cells (gK-/+) entered with entry kinetics similar to those of the KOS virus.
Publication Source (Journal or Book title)
Journal of Virology
First Page
12431
Last Page
12438
Recommended Citation
Foster, T., Rybachuk, G., & Kousoulas, K. (2001). Glycoprotein K specified by herpes simplex virus type 1 is expressed on virions as a Golgi complex-dependent glycosylated species and functions in virion entry. Journal of Virology, 75 (24), 12431-12438. https://doi.org/10.1128/JVI.75.24.12431-12438.2001