Expression of the antimicrobial peptides in plants to control phytopathogenic bacteria and fungi
Three antimicrobial peptides exhibiting in vitro antifungal activity were expressed in Arabidopsis to compare their in planta activity. β-Purothionin, cecropin B, and phor21 were expressed under an endogenous promoter with moderate-level activity and excreted extracellularly. Expression of β-purothionin rendered the greatest antibacterial and antifungal resistance while cecropin B enhanced only antibacterial activity and phor21 did not improve antimicrobial resistance. The transgenic β-purothionin arrested fungal growth on leaf surfaces and infection of stomata. Leaf extracts from plants producing β-purothionin and cecropin B displayed membrane permeabilizing activity. The in planta antimicrobial activity of the tested peptides was consistent with previously reported in vitro experiments. The expression strategy allowed enhanced antifungal resistance without high-level transgene expression. © Springer-Verlag 2005.
Publication Source (Journal or Book title)
Plant Cell Reports
Oard, S., & Enright, F. (2006). Expression of the antimicrobial peptides in plants to control phytopathogenic bacteria and fungi. Plant Cell Reports, 25 (6), 561-572. https://doi.org/10.1007/s00299-005-0102-5