Prowashonupana barley dietary fibre reduces body fat and increases insulin sensitivity in Caenorhabditis elegans model
© 2015 Elsevier Ltd. Prowashonupana barley (PWB) is high in β-glucan with moderate content of resistant starch. PWB reduced intestinal fat deposition (IFD) in wild type Caenorhabditis elegans (C. elegans, N2), and in sir-2.1 or daf-16 null mutants, and sustained a surrogate marker of lifespan, pharyngeal pumping rate (PPR), in N2, sir-2.1, daf-16, or daf-16/daf-2 mutants. Hyperglycaemia (2% glucose) reversed or reduced the PWB effect on IFD in N2 or daf-16/daf-2 mutants with a sustained PPR. mRNA expression of cpt-1, cpt-2, ckr-1, and gcy-8 were dose-dependently reduced in N2 or daf-16 mutants, elevated in daf-16/daf-2 mutants with reduction in cpt-1, and unchanged in sir-2.1 mutants. mRNA expressions were increased by hyperglycaemia in N2 or daf-16/daf-2 mutants, while reduced in sir-2.1 or daf-16 mutants. The effects of PWB in the C. elegans model appeared to be primarily mediated via sir-2.1, daf-16, and daf-16/. daf-2. These data suggest that PWB and β-glucans may benefit hyperglycaemia-impaired lipid metabolism.
Publication Source (Journal or Book title)
Journal of Functional Foods
Gao, C., King, M., Fitzpatrick, Z., Wei, W., King, J., Wang, M., Greenway, F., Finley, J., Burton, J., Johnson, W., Keenan, M., Enright, F., Martin, R., & Zheng, J. (2015). Prowashonupana barley dietary fibre reduces body fat and increases insulin sensitivity in Caenorhabditis elegans model. Journal of Functional Foods, 18, 564-574. https://doi.org/10.1016/j.jff.2015.08.014