Rapid isolation of DNA for genetic screening of catfishes by polymerase chain reaction

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We evaluated two methods, boiling and microwave irradiation, for the rapid isolation of DNA from barbel, adipose fin, and blood of channel catfish Ictalurus punctatus. Compared with routine DNA isolation methods, these procedures were fast (microwaving, 2-4 min; boiling, 10-18 min), simple, and inexpensive (about US$0.10/fish). Samples of DNA isolated from barbels of small (mean ± SD, 2.9 ± 1.0 g) and large (290 ± 43 g) fish were of high purity (ratios of absorbances at 260 and 280 nm, A260/A280 = 1.72-1.90) and were between 0.29 and 0.67 µg/µL in concentration. Samples of DNA isolated from barbel, adipose fin, and blood of small fish, by either method, were used successfully for analysis by polymerase chain reaction (PCR). Samples isolated by boiling of barbel and blood from large fish also proved useful for PCR analysis. These DNA isolation procedures would be useful for rapid genetic screening of channel catfish. Removal of the barbel for tissue analysis would also enable direct marking of fish, and after analysis, individuals designated for further study could be identified. © 1994 Taylor & Francis Group, LLC.

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Transactions of the American Fisheries Society

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