Title

Sequence comparison of sulphonamide resistance genes from Aeromonas salmonicida, Edwardsiella ictaluri, and Escherichia coli

Document Type

Article

Publication Date

12-1-1998

Abstract

Isolates of Aeromonas salmonicida, Edwardsiella ictaluri, and Escherichia coli 1898, from different hosts and geographical locations, displayed resistance to Romet, a potentiated sulphonamide. This resistance is encoded on a plasmid (∼55 kb) containing sulphonamide resistance genes, sul I or sul II as demonstrated by polymerase chain reaction (PCR) analysis. PCR products from either sul I (∼900 bp) or sul II (∼400 bp) were sequenced and compared with the National Institutes of Health GenBank database sequences for these genes. The results suggest common environmental sources of this resistance factor related to various bacterial species and geographical areas. Sul I PCR fragments from various species were identical. This consensus sequence was 99.8% similar to the GenBank sul I sequence. Sul II PCR fragments from various species were 95-100% similar, which was 98.5% similar to the GenBank sul II sequence. The above bacterial species possess nearly identical sul I and sul II gene fragments (>95% homology) to GenBank sul I and sul II sequences from a variety of bacterial species representing hosts and geographical locations other than those in the present study.

Publication Source (Journal or Book title)

Biomedical Letters

First Page

31

Last Page

39

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