The use of male-specific chromosomal DNA fragments to determine the sex of bovine preimplantation embryos
The ability to determine the sex of embryos prior to transfer to recipients has commercial application in the embryo transfer industry. A number of methods of sex determination have been attempted; however, none of these has been commercially successful to date. The identification of repetitive, male-specific bovine chromosomal DNA fragments enables the use of DNA-probe technology in determining the sex of preimplantation embryos from a small embryonic sample. The identification and isolation of three repetitive, male-specific bovine chromosomal DNA fragments, and the application of these probes in a sensitive and highly accurate embryo sexing assay are reported. © 1989.
Publication Source (Journal or Book title)
Bondioli, K., Ellis, S., Pryor, J., Williams, M., & Harpold, M. (1989). The use of male-specific chromosomal DNA fragments to determine the sex of bovine preimplantation embryos. Theriogenology, 31 (1), 95-104. https://doi.org/10.1016/0093-691X(89)90567-0