Re-examination of the role of the Brucella melitensis HtrA stress response protease in virulence in pregnant goats

Document Type


Publication Date



Based on previously reported studies describing the experimental infection of pregnant goats with B. melitensis strain RWP5, we proposed that the HtrA protease plays an important role in the virulence of B. melitensis in its natural ruminant host. Subsequent studies, however, have shown that RWP5 is actually an htrA cycL double mutant. In order to definitively evaluate the role of the B. melitensis htrA in virulence, we constructed an authentic htrA mutant and examined this strain in pregnant goats. The findings of these studies indicate that the contribution of the htrA gene product to the virulence of B. melitensis in its natural host is not as great as was previously proposed. © 2001 Elsevier Science B.V.

Publication Source (Journal or Book title)

Veterinary Microbiology

First Page


Last Page


This document is currently not available here.