Identifier

etd-07022013-114311

Degree

Master of Science (MS)

Department

School of Nutrition and Food Sciences

Document Type

Thesis

Abstract

Cardiovascular disease is the leading cause of death in the US, and circulating free fatty acids (FFAs) are known risk factors associated with cardiovascular inflammation. The influence of 14 dietary FFAs (including saturated, mono-, poly-unsaturated and trans), on the expression of inflammatory markers in human coronary arterial smooth muscle (HACSM) and endothelial (HCAEC) cells using a cell culture model was investigated. HACSM and HCAEC cell cultures were incubated with 200 μM of each FFA for 8 or 24 h respectively at 37 °C in a 5% CO2 humidified incubator. Inflammatory biomarkers were assessed by ELISA or Western Blot in the supernatant or cell lysates respectively. Results showed significant differences in the expression of inflammatory biomarkers among the fatty acid treatments and the control, with myristic and palmitic acids being identified as the most and linoleic acid as the least pro-inflammatory. This confirms that FFAs can induce low-grade inflammation in human coronary arterial cells and provides more information on mode of action.

Date

2013

Document Availability at the Time of Submission

Release the entire work immediately for access worldwide.

Committee Chair

Finley, John W.

Included in

Life Sciences Commons

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