Identifier

etd-11212015-001003

Degree

Master of Science (MS)

Department

Plant Pathology and Crop Physiology

Document Type

Thesis

Abstract

Burkholderia glumae causes bacterial panicle blight in rice by producing the phytotoxin, toxoflavin, as a major virulence factor. Production of toxoflavin is negatively regulated by tepR, which encodes a sigma 54-dependent response regulator. In order to gain insights into the comprehensive biological functions of tepR, the genome-wide transcriptional profile dependent on tepR was analyzed through comparative transcriptome analysis between a tepR defective mutant and the wild-type strain, resulting in the identification of 238 differentially expressed genes (DEGs). Gene Ontology (GO) enrichment analysis indicated that genes involved in flagella assembly and stress response were highly enriched in tepR defective strain. Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment further revealed that tepR positively regulates the gene cluster encoding a type six secretion system (T6SS) and the genes for a branched-chain amino acid ABC Transporter system, but negatively regulates the genes for thiamine, cysteine and methionine metabolism. As homologues of tepR are found in a number of pathogenic Burkholderia species, the modulation of bacterial behavior through the regulatory action of tepR is likely to be a widely occurring phenomenon in this group of bacterial pathogen.

Date

2015

Document Availability at the Time of Submission

Secure the entire work for patent and/or proprietary purposes for a period of one year. Student has submitted appropriate documentation which states: During this period the copyright owner also agrees not to exercise her/his ownership rights, including public use in works, without prior authorization from LSU. At the end of the one year period, either we or LSU may request an automatic extension for one additional year. At the end of the one year secure period (or its extension, if such is requested), the work will be released for access worldwide.

Committee Chair

Ham, Jong Hyun

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