Identifier

etd-07082013-094604

Degree

Master of Science in Mechanical Engineering (MSME)

Department

Mechanical Engineering

Document Type

Thesis

Abstract

The main objective of this work is to study the liposomal uptake of silver and old nanoparticles. Liposomes were prepared in Heating Method. The phospholipids used to prepare liposomes are 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (16:0 PC); 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (16:0 PG); 1,2-distearoyl-sn-glycero-3-phosphocholine (18:0 PC); 1,2-distearoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (18:0 PG).Four different combinations of phospholipids were used to prepare liposomes. In all four combinations two types of phospholipids were used. The liposomes were incubated for 30 minute, 1 hour, 2 hour and 4 hour with silver and gold nanoparticles in streptavidin coated glass slide. All four liposomal formulations had a biotinylated lipid (1-oleoyl-2-[12-biotinyl(aminododecanoyl)]-sn-glycero-3-phosphocholine) which has a string binding affinity for streptavidin . As a result biotinylated liposomes were attached to the streptavidin coated glass slide. After incubation the slides were gently washed with water to get rid of unattached nanoparticles and liposomes. Then the slides were examined under microscope to study the uptake. Uptake was calculated as percentage of liposomes showing nanoparticles attached to them out of total number of liposomes. Liposomes prepared from 16PC & 16PG showed highest amount of uptake while 18PC & 18PG liposomes had lowest uptake for both type of nanoparticles. The uptake was slightly higher for the silver nanoparticles for all four types of liposomes than that of gold nanoparticles. Concentration of the silver nanoparticles was almost 25 times higher than the gold nanoparticle concentration. This suggests that the effect of nanoparticle concentration on the uptake was not much strong. In all cases the uptake was nonlinear function of incubation time. At the beginning the uptake rate was higher which decreased over time. Liposomes prepared from 16PC & 16PG lipids can be great carrier for silver and gold nanoparticle based drug delivery carrier.

Date

2013

Document Availability at the Time of Submission

Release the entire work immediately for access worldwide.

Committee Chair

Devireddy, Ram

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