Date of Award

1997

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

School of Nutrition and Food Sciences

First Advisor

Donal F. Day

Abstract

Glucooligosaccharides of less than DP 10 were produced either by mixed cultures of Leuconostoc mesenteroides ATCC 13146 and L. starkeyi ATCC 74054, or by an acceptor reaction produced by Leuconostoc mesenteroides ATCC 13146. The yields of glucooligosaccharides produced by mixed culture and acceptor reaction reached up to 49 and 90% of theoretical yield. The composition of carbohydrates at the end of fermentation by mixed culture was 31.4% oligosaccharides, 17.6% dextran, 31.4% mannitol, 2.1% fructose, and 2.4% of sucrose. The composition of carbohydrates at the end of fermentation by acceptor reaction was 32.7% pannose, 30.4% branched isomaltotetraose (DP 4), 21.7% branched DP 5, 5.2% branched DP 6, and 3.6% branched DP 7, and 6.5% maltose. The oligosaccharides produced suppressed growth of Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, and Clostridium perfringenes. Bifidobacterium bifidum and Bifidobacterium fullorum completely utilized the oligosaccharides as carbon sources in 48 hr.

ISBN

9780591724318

Pages

131

Share

COinS