Date of Award

1990

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Biological Sciences

First Advisor

Ronald J. Siebeling

Abstract

Vibrio anguillarum exhibits species-specific antigens on the protein core of the polar flagellum (H) and H-determinants which are expressed by heterologous Vibrio species. Monoclonal antibody (MAb) which reacted with flagella core protein by ELISA were affinity purified from protein A-sepharose. Staphylococcus aureus Cowan 1 cells armed with anti-H MAb coagglutinated each of the 10 V. anguillarum O-antigen serovars within 1 to 2 minutes, as well as V. ordalii isolates. These findings suggest these two vibrios express similar if not identical species-specific H-determinants. The anti-H reagent did not coagglutinate 20 heterologous Vibrio species. MAb generated against LPS, extracted from V. anguillarum serovar 01, 02 and 03, were tested for serovar-specificity by ELISA and direct slide agglutination. Anti-02 serovar-specific MAb fixed to S. aureus cells detect the 02 serovar in enrichment culture fluid 6 hours after the broth was seeded with kidney, liver, spleen and blood from diseased fish. Utilization of the anti-O serovar specific MAb coagglutination assay is rapid and applicable to the identification of V. anguillarum from diseased fish. Anti-H can be used to detect potential environmental pathogenic strains which are nontypable with available anti-O sera.

Pages

157

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