Date of Award

1990

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

School of Animal Science

First Advisor

Robert A. Godke

Abstract

Growth characteristics for fetal bovine uterine fibroblast cells (FBUFC) and frozen-thawed bovine (b) and caprine (g) trophoblastic vesicles (TV) were similar in (A) Ham's F-10 (HF-10), (B) MEM (with Earle's salts), (C) RPMI-1640 and (D) Menezo's B$\sb2$ (B$\sb2$), each supplemented with 10% FCS and Ab-Am (Experiment (Exp) I). Cell numbers declined initially but increased linearly thereafter for 5 days. The bTV and gTV that survived freezing had similar viability across time and Treatments (Trt). Exp II compared the (A) bTV, (B) FBUFC monolayer and (C) bTV-monolayer co-culture systems to (D) HF-10 alone for culture of 8- to 32-cell bovine embryos. The embryos (%) considered viable at 48, 72, and 96 hours were: 80, 80, 83 and 78, 80, 73, 51 and 46, 27, 51, 41 and 5 for Trt A, B, C and D, respectively. Trt A, B and C had more viable (P $<$.05) and grade 1 and 2 (P $<$.05) embryos at 96 hours than Trt D. The number of hatched blastocysts was greater (P $<$.05) in Trt B and C than in A and D. Exp III Trt were identical to Exp II except that a peptide from trophoblastic tissue was substituted in Trt A and C. Viable embryos (%) at 48, 72 and 96 hours were 55, 67, 55 and 39, 33, 55, 55 and 33 and 11, 44, 50 and 33 for Trt A, B, C and D, respectively. Grater numbers of viable (P $<$.05) and transferable quality (P $<$.05) embryos were in Trt B and C than in A and D. More embryos hatched in Trt B and C than in Trt A and D. Lipid and free fatty acid (FFA) production by bTV, FBUFC, FBUC, (fetal bovine uterine cells), BOC and GOC (bovine and goat oviduct cells, respectively) compared with B$\sb2$ medium were evaluated (Exp IV). Phospholipid and triglyceride production was similar to that found in B$\sb2$. FFA identified were C14-C20 and four unknowns. Arachidonic acid metabolites (prostaglandins;PGF) produced by early bovine and caprine embryos was determined (Exp V). Bovine and caprine embryos produced 6-keto-PGF$\sb{1a}$, PGF$\sb{2a}$, PGE$\sb2$ and PGFM.

Pages

144

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