Date of Award

1989

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

First Advisor

Auttis M. Mullins

Abstract

Suspected environmental fecal coliform positive Klebsiella sp. oyster isolates were analyzed and compared to human clinical isolates of Klebsiella pneumoniae. Oyster isolates were urease negative (at 24 hours) and were identified as K. pneumoniae using API 20E biochemical test strips. The ability to produce fecal coliform reactions was lost by 65% of the oyster isolates after undergoing several passages on brain-heart-infusion agar at 20$\sp\circ$ to 25$\sp\circ$C. Tests with 4-methylumbelliferyl-B-d-glucuronide (MUG) found both oyster and clinical isolates to be MUG negative. Moles % guanine plus cytosine (mol% G + C) information indicated that oyster isolates had mol% G + C values similar to K. pneumoniae, and DNA-DNA homology information indicated conclusively that chromosomal DNA from oyster isolates was indistinguishable from that of K. pneumoniae, having homologies of $\geq$63% with a mean of 82%.

Pages

94

Share

COinS