Date of Award

1987

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Abstract

Calli from 'Penneagle' and 'Seaside' creeping bentgrass (Agrostis palustris Huds.) were selected for enhanced ability to proliferate on media supplemented with 514 mM NaCl. Based on dry matter production and vigor, growth of plants regenerated from NaCl-adapted calli of both cultivars in saline hydroponic nutrient medium, was superior to that of plants regenerated from non-adapted calli. At 257 mM NaCl, plants from NaCl-sensitive calli died or were unable to produce measurable dry matter whereas plants from NcCl-adapted calli grew well. Growth of plants from NaCl-adapted calli was inhibited at 340 mM NaCl. At 514 mM NaCl, resistant plants no longer produced measurable dry matter, although they remained alive. The extractable soluble protein content of NaCl-adapted calli was less than that of non-adapted cultures. Electrophoretic banding patterns of soluble proteins from NaCl-adapted calli were compared with those from non-adapted calli using one dimensional polyacrylamide gel electrophoresis. One particular band at 31.5kd was unique to the NaCl-adapted calli. The intensity of some bands increased, while others decreased with increasing levels of NaCl-adaptation. One generation (30 days of dark incubation) after being transferred to a medium without NaCl, polypeptide banding patterns of NaCl-adapted calli were similar to that of non-adapted calli.

Pages

118

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